Association of nascent polypeptide and transfer ribonucleic acid with 30S ribosomal subunits

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Association of nascent polypeptide and transfer ribonucleic acid with 30S ribosomal subunits.

1. Crude extracts of Escherichia coli programmed in protein synthesis by endogenous mRNA have incorporated amino acids into protein. Analysis of such extracts by sucrose-gradient centrifugation in low Mg(2+) concentration has revealed that 30S ribosomal subunits carry associated radioactive material of which a considerable proportion can be removed from ribosomes by treatment of pre-labelled ex...

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Two-dimensional crystalline sheets of Bacillus stearothermophilus 50S ribosomal subunits containing a nascent polypeptide chain.

Polylysine chains were synthesized on Bacillus stearothermophilus ribosomes in a poly(A)-programmed in vitro system. After separation of the ribosomal subunits by sucrose gradient centrifugation, the polylysine chains (in contrast to the polyphenylalanine chains synthesized in a poly(U) system) reproducibly remained attached to the large ribosomal subunit. It was possible to produce two-dimensi...

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Reassociation of eukaryotic ribosomal subunits. Kinetics of reassociation; role of deacylated transfer ribonucleic acid; effect of cycloheximide.

The reassociation of ribosomal subunits to form 80 S monomers is catalyzed by a rat liver cytosol fraction (G-25) and by the initiation factor MI (formerly called 40 S binding factor). Reassociation requires a template (we have generally used poly(U)) and either aminoacyl-tRNA or deacylated tRNA. Initiation factor-catalyzed formation of 40 S60 S couples can occur in two ways. The first pathway ...

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Recognition of cognate transfer RNA by the 30S ribosomal subunit.

Crystal structures of the 30S ribosomal subunit in complex with messenger RNA and cognate transfer RNA in the A site, both in the presence and absence of the antibiotic paromomycin, have been solved at between 3.1 and 3.3 angstroms resolution. Cognate transfer RNA (tRNA) binding induces global domain movements of the 30S subunit and changes in the conformation of the universally conserved and e...

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ژورنال

عنوان ژورنال: Biochemical Journal

سال: 1973

ISSN: 0306-3283

DOI: 10.1042/bj1360865